Membrane Insertion of a Potassium-Channel Voltage Sensor

  • Tara Hessa
    Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.
  • Stephen H. White
    Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.
  • Gunnar von Heijne
    Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.

書誌事項

公開日
2005-03-04
DOI
  • 10.1126/science.1109176
公開者
American Association for the Advancement of Science (AAAS)

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説明

<jats:p>The mechanism of voltage gating in K+ channels is controversial. The paddle model posits that highly charged voltage-sensor domains move relatively freely across the lipid bilayer in response to membrane depolarization; competing models picture the charged S4 voltage-sensor helix as being shielded from lipid contact by other parts of the protein. We measured the apparent free energy of membrane insertion of a K+-channel S4 helix into the endoplasmic reticulum membrane and conclude that S4 is poised very near the threshold of efficient bilayer insertion. Our results suggest that the paddle model is not inconsistent with the high charge content of S4.</jats:p>

収録刊行物

  • Science

    Science 307 (5714), 1427-1427, 2005-03-04

    American Association for the Advancement of Science (AAAS)

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