Rabbit Tibial Periosteum and Saphenous Arteriovenous Vascular Bundle as an In Vivo Bioreactor to Construct Vascularized Tissue‐Engineered Bone: A Feasibility Study
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- Dong Han
- Department of Plastic & Reconstructive Surgery Ninth People's Hospital Medical School of Shanghai Jiao Tong University Shanghai China
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- Xiaoyi Guan
- Department of Ophthalmology Luwan Branch Ruijin Hospital Medical School of Shanghai Jiao Tong University Shanghai China
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- Jian Wang
- Department of Hand Surgery Huashan Hospital Affiliated to Fudan University Shanghai China
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- Jiao Wei
- Department of Plastic & Reconstructive Surgery Ninth People's Hospital Medical School of Shanghai Jiao Tong University Shanghai China
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- Qingfeng Li
- Department of Plastic & Reconstructive Surgery Ninth People's Hospital Medical School of Shanghai Jiao Tong University Shanghai China
説明
<jats:title>Abstract</jats:title><jats:p>The aim of this project was to construct vascularized tissue‐engineered living bone with an autologous vascular network by means of a rabbit bioreactor in vivo. The key components of the in vivo bioreactor for bone formation were the vascularized tibial periosteum and the saphenous vascular bundle. Beta‐tricalcium phosphate (β‐<jats:styled-content style="fixed-case">TCP</jats:styled-content>) scaffolds were implanted into the in vivo bioreactor (vascular pedicle implantation and vascularized periosteum encapsulation). At 4 weeks postsurgery, new bone formation was mainly “cartilage‐bone inducing” in the inner periosteum, and was primarily seen in the outer aspects of the scaffold with some amount in the middle part as well. Microvascular infusion showed that direct revascularization of β‐<jats:styled-content style="fixed-case">TCP</jats:styled-content> was obtained by means of vascular implantation. Triple staining results showed a large amount of blue collagen fibers. Vascular endothelial growth factor immunohistochemical staining displayed endothelial cells of new blood vessels in bone tissue. The bioreactor established in this study can be used to prepare tissue‐engineered bone with a vascular network.</jats:p>
収録刊行物
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- Artificial Organs
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Artificial Organs 38 (2), 167-174, 2013-07-11
Wiley