Nuclease‐resistant 63‐bp trimeric si<scp>RNA</scp>s simultaneously silence three different genes in tumor cells
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- Olga V. Gvozdeva
- Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk Russia
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- Daniil V. Gladkih
- Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk Russia
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- Ivan V. Chernikov
- Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk Russia
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- Mariya I. Meschaninova
- Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk Russia
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- Alya G. Venyaminova
- Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk Russia
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- Marina A. Zenkova
- Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk Russia
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- Valentin V. Vlassov
- Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk Russia
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- Elena L. Chernolovskaya
- Institute of Chemical Biology and Fundamental Medicine SB RAS Novosibirsk Russia
抄録
<jats:p>We designed a multimeric nuclease‐resistant 63‐bp trimeric small‐interfering <jats:styled-content style="fixed-case">RNA</jats:styled-content> (tsi<jats:styled-content style="fixed-case">RNA</jats:styled-content>) comprising in one duplex the sequence of si<jats:styled-content style="fixed-case">RNA</jats:styled-content>s targeting <jats:styled-content style="fixed-case">mRNA</jats:styled-content>s of <jats:italic><jats:styled-content style="fixed-case">MDR</jats:styled-content>1</jats:italic>,<jats:italic> <jats:styled-content style="fixed-case">LMP</jats:styled-content>2</jats:italic>, and <jats:italic><jats:styled-content style="fixed-case">LMP</jats:styled-content>7</jats:italic> genes. We show that such tsi<jats:styled-content style="fixed-case">RNA</jats:styled-content> is able to suppress the expression of all the target genes independently and with high efficiency, acting <jats:italic>via</jats:italic> a Dicer‐dependent mechanism. tsi<jats:styled-content style="fixed-case">RNA</jats:styled-content> is diced into 42‐ and 21‐bp duplexes inside the cell. tsi<jats:styled-content style="fixed-case">RNA</jats:styled-content>‐induced gene silencing is characterized by kinetics similar to that of canonical si<jats:styled-content style="fixed-case">RNA</jats:styled-content>, while the silencing efficiency is significantly higher than that of canonical si<jats:styled-content style="fixed-case">RNA</jats:styled-content> with the same sequence.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 592 (1), 122-129, 2017-12-29
Wiley