LncRNA‐MEG3 promotes bovine myoblast differentiation by sponging miR‐135

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  • Mei Liu
    College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling Shaanxi China
  • Bo Li
    College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling Shaanxi China
  • Wenwen Peng
    College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling Shaanxi China
  • Yilei Ma
    College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling Shaanxi China
  • Yongzhen Huang
    College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling Shaanxi China
  • Xianyong Lan
    College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling Shaanxi China
  • Chuzhao Lei
    College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling Shaanxi China
  • Xinglei Qi
    Bureau of Animal Husbandry of Biyang County Biyang Henan China
  • George E. Liu
    Animal Genomics and Improvement Laboratory, BARC, Agricultural Research Service, USDA Beltsville Maryland
  • Hong Chen
    College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling Shaanxi China

Abstract

<jats:title>Abstract</jats:title><jats:p>Long noncoding RNA maternally expressed gene 3 (<jats:italic>lncRNA‐MEG3</jats:italic>) is an important regulator in multiple biological functions. However, <jats:italic>lncRNA‐MEG3</jats:italic>'s function in cattle growth and regulatory mechanism on bovine skeletal muscle development has not yet been well studied. In this project, we first investigated <jats:italic>lncRNA‐MEG3'</jats:italic>s expression profile and detected that it was highly expressed in bovine skeletal muscle tissue and its RNA level was kept increasingly during the early phase of bovine primary myoblast differentiation. Using luciferase reporter assays, we identified the <jats:italic>lncRNA‐MEG3</jats:italic> core promoter containing putative transcription factor binding site for myocyte enhancer factor 2C (MEF2C). Interestingly, we found that <jats:italic>LncRNA‐MEG3</jats:italic> could significantly upregulate and downregulate myosin heavy chain ( <jats:italic>MHC</jats:italic>), myogenin ( <jats:italic>MyoG</jats:italic>), and <jats:italic>MEF2C</jats:italic> through overexpression and RNAi strategies, respectively. Using luciferase reporter assays, we also verified <jats:italic>lncRNA‐MEG3</jats:italic> as a miR‐135 sponge. Overexpression of miR‐135 markedly inhibited the wild type of <jats:italic>lncRNA‐MEG3</jats:italic>, but not the mutant <jats:italic>lncRNA‐MEG3</jats:italic> reporter. The luciferase activity of miR‐135 sensor could be rescued by <jats:italic>lncRNA‐MEG3</jats:italic> via competing for miRNA‐135. In addition, the luciferase activity of <jats:italic>MEF2C</jats:italic> was significantly upregulated by the wild type of <jats:italic>lncRNA‐MEG3</jats:italic>. This study, for the first time, revealed that <jats:italic>lncRNA‐MEG3</jats:italic> could promote bovine skeletal muscle differentiation via interacting with miRNA‐135 and <jats:italic>MEF2C</jats:italic>. The results were valuable for further studies and applications of lncRNA related roles in beef molecular breeding.</jats:p>

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