Identification of the Target of a Transcription Activator Protein by Protein-Protein Photocrosslinking

DOI Web Site 4 Citations
  • Yan Chen
    Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, NJ 08855, USA.
  • Yon W. Ebright
    Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, NJ 08855, USA.
  • Richard H. Ebright
    Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, NJ 08855, USA.

Description

<jats:p> Here it is shown, with the use of protein-protein photocrosslinking, that the carboxyl-terminal region of the α subunit of RNA polymerase (RNAP) is in direct physical proximity to the activating region of the catabolite gene activator protein (CAP) in the ternary complex of the <jats:italic>lac</jats:italic> promoter, RNAP, and CAP. These results strongly support the proposal that transcription activation by CAP involves protein-protein contact between the carboxyl-terminal region of the α subunit and the activating region of CAP. </jats:p>

Journal

  • Science

    Science 265 (5168), 90-92, 1994-07

    American Association for the Advancement of Science (AAAS)

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