Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases
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- Jan B. van Beilen
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
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- Enrico G. Funhoff
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
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- Alexander van Loon
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
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- Andrea Just
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
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- Leo Kaysser
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
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- Manuel Bouza
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
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- René Holtackers
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
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- Martina Röthlisberger
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
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- Zhi Li
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
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- Bernard Witholt
- Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland
抄録
<jats:title>ABSTRACT</jats:title> <jats:p> Several strains that grow on medium-chain-length alkanes and catalyze interesting hydroxylation and epoxidation reactions do not possess integral membrane nonheme iron alkane hydroxylases. Using PCR, we show that most of these strains possess enzymes related to CYP153A1 and CYP153A6, cytochrome P450 enzymes that were characterized as alkane hydroxylases. A vector for the polycistronic coexpression of individual CYP153 genes with a ferredoxin gene and a ferredoxin reductase gene was constructed. Seven of the 11 CYP153 genes tested allowed <jats:italic>Pseudomonas putida</jats:italic> GPo12 recombinants to grow well on alkanes, providing evidence that the newly cloned P450s are indeed alkane hydroxylases. </jats:p>
収録刊行物
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- Applied and Environmental Microbiology
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Applied and Environmental Microbiology 72 (1), 59-65, 2006-01
American Society for Microbiology