A rapid and scalable density gradient purification method for Plasmodium sporozoites

<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Malaria remains a major human health problem, with no licensed vaccine currently available. Malaria infections initiate when infectious <jats:italic>Plasmodium</jats:italic> sporozoites are transmitted by Anopheline mosquitoes during their blood meal. Investigations of the malaria sporozoite are, therefore, of clear medical importance. However, sporozoites can only be produced in and isolated from mosquitoes, and their isolation results in large amounts of accompanying mosquito debris and contaminating microbes.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Here is described a discontinuous density gradient purification method for <jats:italic>Plasmodium</jats:italic> sporozoites that maintains parasite infectivity <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic> and greatly reduces mosquito and microbial contaminants.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>This method provides clear advantages over previous approaches: it is rapid, requires no serum components, and can be scaled to purify >10<jats:sup>7</jats:sup> sporozoites with minimal operator involvement. Moreover, it can be effectively applied to both human (<jats:italic>Plasmodium falciparum</jats:italic>, <jats:italic>Plasmodium vivax</jats:italic>) and rodent (<jats:italic>Plasmodium yoelii</jats:italic>) infective species with excellent recovery rates.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>This novel method effectively purifies viable malaria sporozoites by greatly reducing contaminating mosquito debris and microbial burdens associated with parasite isolation. Large-scale preparations of purified sporozoites will allow for enhanced <jats:italic>in vitro</jats:italic> infections, proteomics, and biochemical characterizations. In conjunction with aseptic mosquito rearing techniques, this purification technique will also support production of live attenuated sporozoites for vaccination.</jats:p> </jats:sec>