GP-2/THP gene family encodes self-binding glycosylphosphatidylinositol-anchored proteins in apical secretory compartments of pancreas and kidney.

DOI Web Site 被引用文献16件
  • S Fukuoka
    Laboratory of Cell and Molecular Biology, Charles A. Dana Research Institute, Thorndike Laboratory, Boston, MA 02215.
  • S D Freedman
    Laboratory of Cell and Molecular Biology, Charles A. Dana Research Institute, Thorndike Laboratory, Boston, MA 02215.
  • H Yu
    Laboratory of Cell and Molecular Biology, Charles A. Dana Research Institute, Thorndike Laboratory, Boston, MA 02215.
  • V P Sukhatme
    Laboratory of Cell and Molecular Biology, Charles A. Dana Research Institute, Thorndike Laboratory, Boston, MA 02215.
  • G A Scheele
    Laboratory of Cell and Molecular Biology, Charles A. Dana Research Institute, Thorndike Laboratory, Boston, MA 02215.

書誌事項

公開日
1992-02-15
DOI
  • 10.1073/pnas.89.4.1189
公開者
Proceedings of the National Academy of Sciences

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説明

<jats:p>A family of homologous genes is shown to encode GP-2, the major glycosylphosphatidylinositol (GPI)-linked glycoprotein of pancreatic zymogen granule membranes, and Tamm-Horsfall protein (THP), a GPI-linked glycoprotein associated with apical vesicles in kidney thick ascending limb of Henle (TALH) cells. The C-terminal regions of GP-2 (Asp54-Phe530) and THP (Asp175-His644) from rat show 53% identity, 86% similarity, and 26 conserved cysteine residues including one epidermal growth factor motif. The unique N-terminal domain of rat THP (unique-THP, Pro29-Gln174) shows four conserved epidermal growth factor motifs, three in tandem and one in reverse orientation. GP-2 homologues are observed in a wide variety of epithelial cells, several of which contain highly regulated secretory processes. GP-2 released from zymogen granule membranes with phosphatidylinositol phospholipase C reacts with anti-cross-reactive determinant antibody (anti-CRD), confirming the GPI nature of the pancreatic homologue. In contrast, GP-2 and THP, released endogenously from pancreas and kidney, respectively, do not react with anti-cross-reactive determinant antibody, suggesting alternative enzymatic mechanisms for their physiological release. Globular domains of GP-2 and THP, but not albumin, show pH- and ion-dependent self-association in vitro. The GP-2/THP family appears to represent a newly discovered class of GPI-anchored proteins, which may utilize pH- and ion-dependent self-association mechanisms for establishing membrane (micro)domains targeted to intracellular secretory compartments.</jats:p>

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