Bone CLARITY: Clearing, imaging, and computational analysis of osteoprogenitors within intact bone marrow

  • Alon Greenbaum
    Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Ken Y. Chan
    Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Tatyana Dobreva
    Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • David Brown
    Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Deepak H. Balani
    Endocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
  • Rogely Boyce
    Comparative Biology and Safety Sciences, Amgen, Thousand Oaks, CA 91320, USA.
  • Henry M. Kronenberg
    Endocrine Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
  • Helen J. McBride
    Comparative Biology and Safety Sciences, Amgen, Thousand Oaks, CA 91320, USA.
  • Viviana Gradinaru
    Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

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Description

<jats:p>Bone clearing and light-sheet microscopy enables visualization and quantification of fluorescent single cells in intact bone.</jats:p>

Journal

  • Science Translational Medicine

    Science Translational Medicine 9 (387), eaah6518-, 2017-04-26

    American Association for the Advancement of Science (AAAS)

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