High performance liquid chromatographic determination of nucleic acid bases in marine environmental samples.

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  • MONTANI Shigeru
    Faculty of Agriculture, Kagawa University
  • TADA Kunio
    Faculty of Agriculture, Kagawa University Research Institute of North Pacific Fisheries, Faculty of Fisheries, Hokkaido University
  • OKAICHI Tomotoshi
    Faculty of Agriculture, Kagawa University

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Other Title
  • 高速液体クロマトグラフィーによる海洋環境試料中の核酸塩基類の定量
  • コウソク エキタイ クロマトグラフィー ニ ヨル カイヨウ カンキョウ シリョ

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Abstract

Purines and pyrimidines are major chemical components of organisms. These bases are thought to play an important role on marine ecosystem and organic geochemistry. Therefore, cleanup procedures were developed for the determination of these bases in marine environmental samples. The separation and determination methods of uracil, thymine, cytosine, adenine, guanine and hypoxanthine were developed by HPLC using cation exchange mode. The present methods were rapid and convenient, as compared with those previously used. The data obtained as total purines and pyrimidines varied from 0.3 to 9.3 vg/l for suspended particles in seawater and 4 to 54 μg/g for sediment core samples obtained from Harima-Nada of Seto Inland Sea in Japan.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 35 (7), 618-621, 1986

    The Japan Society for Analytical Chemistry

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