等濃度法を用いる吸光光度分析　　原理と応用

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書誌事項

タイトル別名
  • Iso-concentration method in spectrophotometry; Principle of the method and its application.
  • トウ ノウドホウ オ モチイル キュウコウ コウド ブンセキ ゲンリ ト オウ
  • Principle of the method and its application
  • 原理と応用

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説明

A new technique, the iso-concentration analysis, has been applied to the spectrophotometry. Two series of aliquots are taken. In the first series, each aliquot contains the same amounts of the substance x= (x, x, …), to be taken to which an incremental amount of the standards is added as x +y = (x+y1, x+y2, …). The second series contains an α times amount of the standards added to the first series, y' (αy 1, αy2…). When all the aliquots of both series are made up to the same volume, V, the concentration of one of the aliquots in the first series is equal to that of second series. To all aliquots is added the constant amount of reagent which reacts with the species to be determined and forms the colored complex. The absorbance in any aliquot of the first and second series is given as Eqs.(1) and (2), respectively.<BR>Ax+y=εkx+y(x+y)/V…(1)<BR>Ay'= εky' αy/V…(2)<BR>where Ax+y and Ay' are the absorbance. ε is the molar absorptivity, and kx+y and ky' are the constants. From both equations one obtains Eq.(3).<BR>αi (kx+y/ky')(1+ (x /y)) =(kx+y/ky')(1 +xz) …(3)<BR>where i =Ax+y/AAy', and z= 1/y. The plots of αi vs. z show an S-shape curve. All the curves of αi =f (z) have a common intersection at the point of kx+y= ky', which shows ik= 1. One obtains Eq.(4).<BR>x=(α-1)/zk=(α-1)yk…(4)<BR>The intersection point can be obtained as the intersection of the curve of αi=f (z) with αi=α. If α = 2, x is equal to yk, i.e., the amount of substance present in the sample can be determined without using a calibration curve. The method was successfully applied to the determination of antimony using a reaction with Brilliant Green.

収録刊行物

  • 分析化学

    分析化学 35 (7), 626-628, 1986

    公益社団法人 日本分析化学会

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