Microdetermination of polyamines (putrescine, cadaverine, spermidine, and spermine) using polyamide thin-layer

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Other Title
  • ポリアミン類（プトレシン，カダベリン，スペルミジン及びスペルミン）のポリアミド薄層を用いた微量定量法
  • ポリアミンルイ プレシン カダベリン スペルミジン オヨビ スペルミン ノ ポ

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Description

A fluorometric method for the determination of polyamines (putrescine, cadaverine, spermidine, and spermine) using polyamide thin-layer is descsibed. Dansylated polyamines obtained after the reaction with dansyl-chloride at pH 9.5 at 40°C for 1 h were extracted into toluene. An aliquot of the extract was spotted on the polyamide thin-layer plate, and developed with a solvent system of water-formic acidtriethylamine (54 : 15 : 2). Spots were measured with a scanning fluorometer. The fluorescence intensity of dansylated polyamines was stable on the plate at least 24 h. The relative standard deviation of the fluorescence intensity obtained on the same thin-layer plate was (1.55.0) % at 200 pmol/spot, and the lowest limit of determination was 10 pmol/spot for bis-DNS-putrescine, bis-DNS-cadaverine, and tri-DNS-spermidine, and 5 pmole/spot for tetra-DNS-spermine. This method was successfully applied for the determination of polyamines in tissues of male rats and guinea pigs.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 31 (11), 624-627, 1982

    The Japan Society for Analytical Chemistry

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