ミニカラム濃縮法を用いた環境水中の直鎖型アルキルベンゼンスルホン酸塩の高速液体クロマトグラフィーによる迅速定量

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タイトル別名
  • Rapid HPLC determination of linear alkylbenzenesulfonates in environmental water by using concentration technique with mini column.
  • ミニ カラム ノウシュクホウ オ モチイタ カンキョウスイチュウ ノ チョクサ

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抄録

Rapid HPLC method is presented to determine trace amounts of linear alkylbenzenesulfonates (LAS) in environmental water samples. An ODS mini-cartridge, equipped on sampling loop line of 6-way switching valve, was used for concentrating LAS in water samples. A 10 ml of water sample was filtrated with a membrane filter disk(Gelman HPLC Disk 25CR), and LAS adsorbed on the disk was washed with two successive 2 ml-portions of methanol. The combined solution of the filtrate and washings, to which 0.5 ml volume of 0.25 M sodium borate buffer (pH 9) was added, was made up to 15 ml with methanol. An aliquot(0.55 ml) of the sample solution was injected into the cartridge. The LAS trapped was purified with 0.5 ml of 50% methanol, and introduced into a sparation column by passing the mobile phase through the cartridge. LC conditions were as follows : separation column, Develosil ODS (5 μm, 4 mm × 20 cm) ; mobile phase, 0.05 M sodium perchlorate-acetonitrile(2 : 3, v/v);detector, fluorescence detector(excitation wavelength 225 nm, emission wavelength 290 nm). LAS in the water samples were identified on the basis of retention times and determined by peak height method. Recoveries of LAS from water samples were 9096% and relative standard deviation were 1.63.6%. The detection limit of LAS by the proposed method was 10 ng. Therefore, limit of determination was 0.003 mg/l, when 5 ml of the sample solution was injected.

収録刊行物

  • 分析化学

    分析化学 36 (4), 276-281, 1987

    公益社団法人 日本分析化学会

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