チオクローム化及びカラム濃縮／蛍光検出による環境水中ビタミンＢ１の高感度迅速分析法

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タイトル別名
  • Rapid and highly sensitive determination of thiamine in environmental water by oxidation to thiochrome and column enrichment/fluorometry.
  • チオクロームカ オヨビ カラム ノウシュク ケイコウ ケンシュツ ニ ヨル

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抄録

Trace thiamine in environmental water was oxidized to fluorescent thiochrome by potassium ferricyanide in an alkaline solution (pH 13). The resulting thiochrom was enriched on a reversed-phase HPLC column (Asahipak ODP-50, 6 mm i.d.×150mm), eluted with methanol-water {1:1(v/v)}, and determined by fluorometry at 430nm (excitation wavelength 375nm). Under the optimum conditions, linear calibration curves were obtained for 010ppt and 0100ppt of thiamine when either 10ml or 20ml sample solution was used. Relative standard deviation obtained in each of 6 measurments at 10ppt and 100ppt was 3.9% and 1.4%, respectively. The detection limit of thiamine was 0.8ppt (S/N=2) and the determination period was approximately one hour. The present method was applied to the determination of dissolved thiamine in lake, pond, river and treated sewage water.

収録刊行物

  • 分析化学

    分析化学 40 (3), 115-118, 1991

    公益社団法人 日本分析化学会

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