High performance liquid chromatographic determination of 1- and 3-methyladenines and <I>O</I><SUP>6</SUP>-methylguanine in deoxyribonucleic acid formed by carcinogenic methylating agents
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- YUKI Hidetaka
- School of Pharmaceutical Science, Toho University
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- IMAMURA Makoto
- School of Pharmaceutical Science, Toho University
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- KAWASAKI Hideki
- School of Pharmaceutical Science, Toho University
Bibliographic Information
- Other Title
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- 発がん性メチル化剤により生成したデオキシリボ核酸中の1‐及び3‐メチルアデニンとO`6´‐メチルグアニンの高速液体クロマトグラフィーによる定量
- ハツガンセイ メチルカザイ ニ ヨリ セイセイシタ デオキシリボ カクサンチュ
- 発がん性メチル化剤により生成したデオキシリボ核酸中の1-及び3-メチルアデニンとO^6-メチルグアニンの高速液体クロマトグラフィーによる定量(<特集>生体成分の高速液体クロマトグラフィー)
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Abstract
A high performance liquid chromatographic determi-nation of 1- and 3-methyladenines and O6-methylguanine in nucleic acid was developed. Thirty milligrams of methylated DNA was dissolved in 30 ml of water and pH of the solution was adjusted to 1.6 with 0.1 M hydrochloric acid. The solution was dialyzed against 400 ml of pH 1.6 hydrochloric acid at 37°C for 26 h. The solution was evaporated under reduced pressure to 10 ml, and 5μl of the concentrate was injected onto a high performance liquid chromatograph. The method was applied for the determination of the DNA bases produced by methylation with methylnitrosourea(MNU) or N-methyl-N'-nitro-N-nitrosoguanidine (in vitro) and of methylated bases in liver DNA of rats treated with dimethylnitrosamine or MNU (in vivo). Each methylated base was successfully detected and deter-mined in all these samples. The lower limits of de-tection of the three methylated bases were 2.5 × 10-3, 5 × 10-3, and 5 × 10-3 % of the parent major bases, adenine or guanine, respectively. The recoveries of the three methylated bases were 96.4, 96.6, and 95.8 %, respectively. These results suggest the mode of reaction of carcinogenic methylating agents on DNA.
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 33 (7), 351-355, 1984
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204051761280
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- NII Article ID
- 110002908971
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- NII Book ID
- AN00222633
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- NDL BIB ID
- 2995766
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- ISSN
- 05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed