ミクロスラブポリアクリルアミドゲル電気泳動法によるタンパク質の高性能-多試料分析法

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タイトル別名
  • High performance-multisample analysis of proteins by micro-slab polyacrylamide gel electrophoresis.
  • ミクロスラブポリアクリルアミドゲル電気泳動法によるたんぱく質の高性能‐多試料分析法
  • ミクロ スラブ ポリアクリルアミドゲル デンキ エイドウホウ ニ ヨル タンパ

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For the rapid analysis of a large number of protein samples, an analytical system using micro-slab polyacrylamide gel electrophoresis was developed. Two sizes of gels were employed, one (38 × 37 × 0.5mm) for 10 samples and the other (38 × 100 × 0.5mm) for 25 samples. Twenty six slab gels for 10 samples or 8 slab gels for 25 samples were prepared in a gel casting chamber in a time. Eighty samples (8 slab gels for 10 samples) or 100 samples (4 slab gels for 25 samples) were analyzed in parallel in one cycle. A piece of filter paper covered by a stainless steel plate was used as a bridge between the buffer on a slab gel and that in the cathode chamber. Electrophoresis was performed at a constant current of 40mA per slab gel for 4min. The gels were stained in 0.1% Coomassie Brilliant Blue R-250 in a mixture of methanol, acetic acid and water (50 : 7 : 43, by volume) for 3 min and destained in 7% acetic acid for 5 min. For the detection of very small quantity of proteins (500 pg), the gels were silver-stained within 15 min. Overall standard analysis time of the present method was 30 min including setting of gel molds, application of samples, electrophoretic run and Coomassie Blue staining. Quantitative analysis of the stained protein bands was performed by television camera-microcomputer system.

収録刊行物

  • 分析化学

    分析化学 34 (4), 151-156, 1985

    公益社団法人 日本分析化学会

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