Liposome immunoassay of anti-asialo-GM1 antibody detected by kinetic method of analysis in flow injection system.

DOI IR NDL Digital Collections Web Site Web Site 2 Citations
  • KATAOKA Masamitsu
    Otaru University of Commerce
  • ABE Hirohisa
    Department of Chemistry, Faculty of Science, Hokkaido University
  • UMEZAWA Yoshio
    Department of Chemistry, Faculty of Science, Hokkaido University
  • YASUDA Tatsuji
    Department of Cell Chemistry, Institute of Cellular and Molecular Biology, Okayama University Medical School

Bibliographic Information

Other Title
  • リポソームを用いる抗アシアロＧＭ１抗体の免疫測定へのフローインジェクション／接触分析法の応用
  • リポソーム オ モチイル コウ アシアロ GM1 コウタイ ノ メンエキ ソク
  • リポソームを用いる抗アシアロGM_1抗体の免疫測定へのフローインジェクション/接触分析法の応用(<特集>表面・界面・薄膜と分析化学)

Search this article

Abstract

An immunoassay technique using an immunoreaction at a liposome membrane surface is described. Multilamellar liposomes comprised dipalmitoylphosphatidylcholine(DPPC), cholesterol(Chol) and anti-asialo-GM1(GA1) antigen in the molar ratio 1:1:0.1. Molybdate ions entrapped in the liposomes as marker ions were released from the liposomes by a complement-mediated immunoreaction, and acted as a catalyst for promoting the hydrogen peroxide-iodide ion redox reaction. The most suitable pH of the kinetic reaction and concentrations of hydrogen peroxide and sodium iodide for the determination of molybdate ion were found to be 1, 4.7×10-3 and 1.0×10-3M, respectively. To minimize the sample volume, a flow-injection method was adopted. The immunoreaction was carried out as follows. A mixed solution of sample anti-GA1 antibody, liposomes and complement were incubated at 37.5°C for 1 h. The resulting solution was injected into the kinetic-FIA system. The decrease in the number of iodide ions by the molybdate ion-catalyzed reaction was monitored using an iodide ion-selective electrode. The marker ion release was specific for the anti-GA1 antibody, and depended on the presence of a complement. The present method can be used to detect as low as 103 to 104 dilution of anti-GA1.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 40 (11), 697-703, 1991

    The Japan Society for Analytical Chemistry

Citations (2)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top