Direct determination of high-density lipoprotein- and total cholesterol in serum using a peroxidase-entrapped electrode and polyethylene glycol-modified enzymes.

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  • ペルオキシダーゼ固定電極及びポリエチレングリコール修飾酵素を用いる血清中高密度リポタンパクコレステロール及び総コレステロールの直接測定
  • ペルオキシダーゼ コテイ デンキョク オヨビ ポリエチレン グリコール シュウ

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Abstract

The total concentrations of lipoprotein (LP) cholesterol (CR) in serum were directly determined by amperometric measurements of H2O2 generated by polyethylene glycol (PEG)-modified cholesterol esterase and cholesterol oxidase at a membrane-covered, peroxidase-entrapped and ferrocene-embedded carbon-paste electrode. The concentrations of LP aggregators, such as sodium dextran sulfate and MgCl2, and the amount of the PEG-modified enzymes were optimized to determine the high-density lipoprotein (HDL)-CR concentration. The characteristics of the discrimination between HDL and other LPs by the PEG-modified enzymes and the aggregators are discussed. Using a surfactant as a solubilizer of LP aggregation, the HDL-and total CR concentrations have been discriminatively determined. This method was applied to the determination of the HDL-CR values of 17 human samples. The evaluated values were well correlated to those determined by a commercial spectrophotometric determination kit using the PEG-modified enzymes and also by an amperometric determination of the HDL-fraction prepared by an ordinary precipitation method.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 47 (4), 233-238, 1998

    The Japan Society for Analytical Chemistry

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