Advances of indirect photometric detection in liquid chromatography(Review).

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  • 液体クロマトグラフィーにおける間接吸光度検出法の新展開
  • エキタイ クロマトグラフィー ニ オケル カンセツ キュウコウド ケンシュツホ

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Description

New photometric detection methods developed for use in liquid chromatography have been reviewed. Differential photometric detection using UV-visible absorbing modifiers enabled us to examine the retention and detection mechanism of not only analytes but also mobile phases. Indirect photometric detection ion chromatography based on this theory separately and sensitively determines ionic compounds using a conventional highperformance liquid chromatographic system with a UV-visible absorbance detector. This method is useful for many kinds of samples such as environmental water, foods and biological materials. Polarized photometric detection methods with two polarizers on either side of a UV-visible absorbance detector flow cell have been developed. The monitor determines optically active compounds as the change in absorbance. This method was effectively applied for determining sugars and organic acids.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 44 (6), 417-436, 1995

    The Japan Society for Analytical Chemistry

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