Flow injection analysis for sugars in foods using Titanium(IV)-porphyrin reagent.

  • YOKOI Yuta
    School of Pharmacy, Tokyo University of Pharmacy and Life Science
  • MATSUBARA Chiyo
    School of Life Science, Tokyo University of Pharmacy and Life Science
  • TAKAMURA Kiyoko
    School of Pharmacy, Tokyo University of Pharmacy and Life Science

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Other Title
  • チタン(IV)‐ポルフィリン錯体試薬を用いる食品中糖類のフローインジェクション分析
  • チタン 4 ポルフィリン サクタイ シヤク オ モチイル ショクヒンチュウ ト
  • チタン(IV)-ポルフィリン錯体試薬を用いる食品中糖類のフローインジェクション分析

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Abstract

A rapid and sensitive flow injection analysis (FIA) with spectrophotometric detection by a titanium(IV)-porphyrin reagent was examined for the continuous determination of glucose, sucrose, maltose and lactose in foods using immobilized enzyme reactors to yield hydrogen peroxide. A sample solution is carried by valve to three hydrolysis enzyme reactors in the flow system. The reagent is an aqueous solution of oxo[5, 10, 15, 20-tetra(4-pyridyl)porphyrinato]titanium(IV), TiO(tpypH4)4+, which reacts with hydrogen peroxide to form a monoperoxo complex, TiO2(tpypH4)4+, having an absorption peak at 450nm. The hydrogen peroxide produced from each sugar was determined by combining the reactions with glucose oxidase (GOD) and hydrolytic enzyme reactors corresponding to each sugar. Linear relations were observed between the peak heights and sugar concentrations in samples containing 0.5500μM (1010000 pmol/test) glucose, and 11000 μM (2020000 pmol/test) sucrose, maltose and lactose. The relative standard deviations for ten replicate determinations of glucose, sucrose, maltose and lactose were 0.74, 0.75, 0.84 and 0.49%, respectively, for each standard solution of 100μM. The method permitted analysis of 20 kinds of sugar in samples per hour. This method was applied to determining these four sugars in foods such as milk, soft drinks and wine with satisfactory results.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 44 (5), 355-362, 1995

    The Japan Society for Analytical Chemistry

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