High performance liquid chromatography of aliphatic amines by using resonance Raman detection.

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  • 共鳴ラマン検出法による脂肪族アミンの高速液体クロマトグラフィー
  • キョウメイ ラマン ケンシュツホウ ニ ヨル シボウゾク アミン ノ コウソク

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Abstract

A high performance liquid chromatography (HPLC)with a Raman spectrometric detector is described. In order to obtain high sensitivity, utilization of the resonance Raman effect was attempted. Seven 4-dimethylaminoazobenzene-4'-sulfone amides were derivatized from the reactions of 4-dimethylaminoazobenzene-4'-sulfonyl chloride with primary and secondary amines. These derivatives were separated successfully with a Zorbax ODS column (4.6φ×250mm). A mixed solvent of 70% (v/v) acetonitrile/water was used as a mobile phase. Chromatogram was obtained by measuring the intensity of resonance Raman scattering at 1136cm-1 with 488.0nm line of Ar+laser at 300mW output. A flow cell (0.9mm i.d.) was employed and its effective cell volume was 3.7nl. A detection limit for the methylamine derivative was 53.8ng. As the flow cell has a micro volume, this method will be applicable to HPLC of micro or semi-micro scale.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 34 (2), 63-67, 1985

    The Japan Society for Analytical Chemistry

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