体液中微量胆汁酸の固相抽出(<特集>超微量分析のための前処理と予備濃縮)

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タイトル別名
  • Solid phase extraction of bile acids in biological fluids.
  • 体液中微量胆汁酸の固相抽出
  • タイエキチュウ ビリョウ タンジュウサン ノ コソウ チュウシュツ

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Comparative studies on solid-phase extraction for the determination of bile acids in biological fluids were carried out. A test sample containing various amounts of bile acids was passed through a column of Amberlite XAD-2 resin or cartridge packed with octadecylsilyl (ODS) bonded silica, i.e., Bond Elut and Sep-Pak C18. Bile acids were eluted, derivatized with 1-anthroyl nitrile through their 3α-hydroxyl groups and determined by HPLC with fluorescence detection. When 2.0 nmol each of bile acids were applied to the Amberlite XAD-2 column, the recovery rates were insufficient, in particular for conjugated bile acids. On the other hand, when more than 20 nmol each of bile acids were loaded, the satisfactory results were obtained. The use of Bond Elut and Sep-Pak C18 cartridges provided almost the quantitative recovery of bile acids, even when 0.2 nmol each of bile acids were applied. It has proved that Amberlite XAD-2 resin is applicable to bile specimens for solid-phase extraction, while the cartridge packed with ODS bonded silica is also useful for serum sample preparation.

収録刊行物

  • 分析化学

    分析化学 36 (11), 772-775, 1987

    公益社団法人 日本分析化学会

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