High speed liquid chromatographic analysis of isopropylmethylphenol in cosmetic products

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Other Title
  • 高速液体クロマトグラフィーによる化粧品中のイソプロピルメチルフェノールの分析
  • コウソク エキタイ クロマトグラフィー ニヨル ケショウヒンチュウ ノ イソプ

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Description

High speed liquid chromatographic analysis of isopropylmethylphenol (3-methyl-4-isopropylphenol) on Nucleosil 5NH2 (5μm, Macherey-Nagel) was studied. The condition was as follows; column size: 4mm i.d.×250mm, detector: fluorescence spectrophotometer (exciting wavelength: 280nm and fluorescence wavelength: 305nm), eluent: 10% ethanol- hexane, flow rate: 1.16ml/min. column temperature: 40°C.Determination was carried out successfully by using thymol or estradiol as an internal standard. The plots of peak height ratio vs. amount ratio (isopropylmethylphenol-thymol or estradiol) were linear between 2.5ppm to 12.5ppm. The experimentally prepared hair tonic and cream were diluted (1:100) with ethanol and dehydrated with anhydrous sodium sulfate. Thymol (5ng/μl) or estradiol (20ng/μl) was incorporated into this mixture as an internal standard. The averaged recoveries from hair tonic and cream ranged 100.5% to 100.6% and c. v. (%) were 1.25 to 1.02, respectively. This method is very simple, accurate and selective, therefore it is applicable to determine isopropylmethylphenol in cosmetic products at routin analyses.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 29 (4), 272-275, 1980

    The Japan Society for Analytical Chemistry

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