Determination of aliphatic aldehydes in milk, milk products, edible fat and oil by HPLC.
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- HIROTA Tetsuji
- Food Research Laboratory Quality Assurance Dept., Snow Brand Milk Products Co.
Bibliographic Information
- Other Title
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- 高速液体クロマトグラフィーによる乳・乳製品及び食用油脂中のアルデヒド類の定量
- コウソク エキタイ クロマトグラフィー ニヨル ニュウ ニュウセイヒン オヨビ
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Abstract
A sensitive HPLC method with postcolumn-labeling using 2, 4-dinitropheriylhydrazine (DNPH) was developed for the determination of aliphatic aldehydes in milk, milk products, edible fat and oil. An ethanolic sample solution was prepared by mixing 10g of a food with 50 ml of ethanol. A reaction mixture was prepared by mixing 90 ml of ethanol, 2 ml of hydrochloric acid, 50 mg of DNPH and 8 ml of water. One milliliter of the sample solution was added to 1 ml of the reaction mixture. The mixture was allowed to stand at 45°C for 30 min to form aliphatic aldehydes-DNPH derivatives. Twenty microliters of the reactant were injected into an HPLC system and separated in a reversed-phase column with an aqueous solution of acetonitrile (water : acetonitrile=80:25). Aliphatic aldehydes-DNPH were completely separated in 25 min by HPLC. The detection limits, monitored at 365 nm, were about 0.040.2μg for each of the aliphatic aldehydes in 1 g of sample. The reproducibility of the HPLC measurements was 0.54.9% (RSD). This method was successfully applied to the determination of aliphatic aldehydes in milk, milk products, edible fat and oil.
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 46 (1), 31-36, 1997
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001204053396864
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- NII Article ID
- 110002907336
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- NII Book ID
- AN00222633
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- NDL BIB ID
- 4118297
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- ISSN
- 05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed