高速液体クロマトグラフィーによる乳・乳製品及び食用油脂中のアルデヒド類の定量

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タイトル別名
  • Determination of aliphatic aldehydes in milk, milk products, edible fat and oil by HPLC.
  • コウソク エキタイ クロマトグラフィー ニヨル ニュウ ニュウセイヒン オヨビ

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A sensitive HPLC method with postcolumn-labeling using 2, 4-dinitropheriylhydrazine (DNPH) was developed for the determination of aliphatic aldehydes in milk, milk products, edible fat and oil. An ethanolic sample solution was prepared by mixing 10g of a food with 50 ml of ethanol. A reaction mixture was prepared by mixing 90 ml of ethanol, 2 ml of hydrochloric acid, 50 mg of DNPH and 8 ml of water. One milliliter of the sample solution was added to 1 ml of the reaction mixture. The mixture was allowed to stand at 45°C for 30 min to form aliphatic aldehydes-DNPH derivatives. Twenty microliters of the reactant were injected into an HPLC system and separated in a reversed-phase column with an aqueous solution of acetonitrile (water : acetonitrile=80:25). Aliphatic aldehydes-DNPH were completely separated in 25 min by HPLC. The detection limits, monitored at 365 nm, were about 0.040.2μg for each of the aliphatic aldehydes in 1 g of sample. The reproducibility of the HPLC measurements was 0.54.9% (RSD). This method was successfully applied to the determination of aliphatic aldehydes in milk, milk products, edible fat and oil.

収録刊行物

  • 分析化学

    分析化学 46 (1), 31-36, 1997

    公益社団法人 日本分析化学会

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