Modification of a light scattering detector for supercritical fluid chromatography.

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Other Title
  • 超臨界流体クロマトグラフィー用光散乱検出器の試作
  • チョウリンカイ リュウタイ クロマトグラフィーヨウ ヒカリ サンラン ケンシュ

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Abstract

A commercially available portable dust meter was modified for use as a light scattering detector (LSD) for supercritical fluid chromatography (SFC). The sample introduction port of the meter was replaced by a specially designed interface which was connected to the supercritical fluid chromatograph. This connector consisted of a nebulizer tube of a fused silica capillary tube (i. d. 50μm) and a heater tube over the nebulizer. It was connected to a packed column (ODS, 5μm, i. d. 4.6mm×250mm) and was operated at inlet pressure of 180kg/cm2 and temperature of 50°C. The temperature of the heater tube was set at 50°C. The samples evaluated were choresterol, butter and margarine extracts. After examining the optimal conditions using cholesterol as the sample, chromatograms of the extracts were obtained together with those using UV detector. Addition of a modifier to the mobile carbon dioxide phase did not give any interference. Though the LSD has some problem with spike noise and repeatability yet, it proved to be an universal detector for SFC.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 40 (3), 131-135, 1991

    The Japan Society for Analytical Chemistry

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