Effects of High Pressure on Protein Crystallization
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- SAZAKI Gen
- Institute for Materials Research, Tohoku University Center for Interdisciplinary Research, Tohoku University
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- NAKAJIMA Kazuo
- Institute for Materials Research, Tohoku University
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- SUZUKI Yoshihisa
- Department of Chemical Science and Technology, The University of Tokushima
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- TAMURA Katsuhiro
- Department of Chemical Science and Technology, The University of Tokushima
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- MIYASHITA Satoru
- Physics, Toyama Medical and Pharmaceuticall University
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- SAWADA Tsutomu
- National Institute for Materials Science
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- KOMATSU Hiroshi
- Faculty of Policy Studies, Iwate Prefectural University
Bibliographic Information
- Other Title
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- タンパク質の結晶化に及ぼす圧力効果
- サイキン ノ ケンキュウ カラ タンパクシツ ノ ケッショウカ ニ オヨボス アツリョク コウカ
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Abstract
Hydrostatic pressure is a useful parameter to control protein crystallization. One can change the solubility of protein crystals quickly by changing pressure. Information about the hydration of the specific surfaces of protein molecules can be obtained from the pressure dependency of solubility. When a protein molecule is hydrophobic or when its molecular volume in a solution is significantly larger than that in a crystal, applying high pressure is expected to be an effective method to promote crystallization. Pressure also affects the growth kinetics of protein crystals. Dependency of a growth rate on a driving force for crystallization was measured on typical proteins and analyzed using a two-dimensional nucleation growth model of a poly-nuclei type. High-pressure effects on growth kinetics could be explained by changes in a surface free energy, an activation energy and an average distance between kinks. Common understanding, however, has not yet been obtained for growth kinetics.
Journal
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- Nihon Kessho Gakkaishi
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Nihon Kessho Gakkaishi 46 (2), 152-158, 2004
The Crystallographic Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204085842432
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- NII Article ID
- 10012907347
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- NII Book ID
- AN00188364
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- ISSN
- 18845576
- 03694585
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- NDL BIB ID
- 6937401
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed