Crystal Structure Analysis of Calcium Pump from Sarcoplasmic Reticulum.
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- NAKASAKO Masayoshi
- Institute of Molecular and Cellular Biosciences, The University of Tokyo
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- NOMURA Hiromi
- Institute of Molecular and Cellular Biosciences, The University of Tokyo
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- OGAWA Haruo
- Institute of Molecular and Cellular Biosciences, The University of Tokyo
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- TOYOSHIMA Chikashi
- Institute of Molecular and Cellular Biosciences, The University of Tokyo
Bibliographic Information
- Other Title
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- 筋小胞体カルシウムポンプの結晶構造解析
- カイセツ キン ショウホウタイ カルシウム ポンプ ノ ケッショウ コウゾウ カイセキ
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Abstract
Calcium ATPase (Calcium pump) from sarcoplasmic reticulum is an integral membrane protein of Mr 110 K and a representative member of P-type ATPase involved in the active transports of ions with the energy from ATP hydrolysis. This ion pump regulates muscle relaxation by up-taking calcium ions from muscle cells into sarcoplasmic reticulum against the concentration gradient of the calcium. This ion pump has been successfully crystallized into ultra-thin plate crystals (<20 micron) by a dialysis method. Through the multiple-isomorphous replacement experiments at cryogenic temperature, the three-dimensional structure of this pump has been determined at a resolution of 2.6Å. The crystal structure is the first to reveal the structural architecture of P-type ATPase. Here, we describe the methods used for solving the phase problem in the crystal structure analysis for such ultra-thin plate crystal: combination of cryogenic X-ray diffraction experiments and cryogenic electron microscopy.
Journal
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- Nihon Kessho Gakkaishi
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Nihon Kessho Gakkaishi 42 (6), 478-485, 2000
The Crystallographic Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204086819328
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- NII Article ID
- 10007410631
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- NII Book ID
- AN00188364
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- ISSN
- 18845576
- 03694585
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- NDL BIB ID
- 5611195
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed