Control of Protein Crystallization Using Laser Irradiation

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  • ADACHI Hiroaki
    Department of Electrical Engineering, Graduate School of Engineering, Osaka University
  • TAKANO Kazufumi
    Department of Electrical Engineering, Graduate School of Engineering, Osaka University
  • MATSUMURA Hiroyoshi
    Department of Electrical Engineering, Graduate School of Engineering, Osaka University
  • INOUE Tsuyoshi
    Department of Electrical Engineering, Graduate School of Engineering, Osaka University
  • MORI Yusuke
    Department of Electrical Engineering, Graduate School of Engineering, Osaka University
  • SASAKI Takatomo
    Department of Electrical Engineering, Graduate School of Engineering, Osaka University

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Other Title
  • レーザ照射による蛋白質結晶成長制御
  • サイキン ノ ケンキュウ カラ レーザー ショウシャ ニ ヨル タンパクシツ ケッショウ セイチョウ セイギョ

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Abstract

We developed crystallization method of a protein molecule by laser irradiation, and call this process Laser Irradiated GrowtH Technique (LIGHT) . Effective crystallization was confirmed by applying a femtosecond laser. The crystallization period was shortened by LIGHT. Protein crystals were also obtained by LIGHT from normally uncrystallized conditions. These results indicate that laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. We should optimize the laserirradiated condition to control the number of crystals and to obtain high-quality crystals because protein crystallization depends on the conditions.

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