An Insight into the Mechanism of Enzymatic Reaction on Aminoacyl-tRNA Synthetases

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  • アミノアシル‐ｔＲＮＡ合成酵素の酵素反応機構に関する研究
  • アミノアシル-tRNA合成酵素の酵素反応機構に関する研究
  • アミノアシル tRNA ゴウセイ コウソ ノ コウソ ハンノウ キコウ ニ カンスル ケンキュウ

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Abstract

The mechanism that ATP-pyrophosphate exchange reaction catalyzed by Arg-tRNA, Gln-tRNA and Glu-tRNA synthetases requires assistance of the cognate tRNA, remained to be established. On the basis of crystal structure of Arg-tRNA synthetase from Pyrococcus horikoshii complexed with tRNAArgCCU and ATP analog, we constructed a structural model for a mechanism in which formation of a hydrogen bond between 2’-OH group of A76 of tRNA and carboxyl group of Arg induces both formation reaction of Arg-AMP from Arg and ATP and the reverse reaction pyrophosphorolysis at low pH. The hydrogen bond assists also editing of misacylated Thr-tRNAVAl or Thr-AMP bound Val-tRNA synthetase.

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