書誌事項
- タイトル別名
-
- Structural Determination for Switching between the Polymerase and Exonuclease Modes in the PCNA-Replicative DNA Polymerase Complex
- DNA クランプ フクセイ DNA ポリメラーゼ フクゴウタイ ニ オケル DNA ゴウセイ シュウセイ スイッチング キコウ
この論文をさがす
抄録
Proliferating cell nuclear antigen (PCNA) is responsible for the processivity of DNA polymerase. We determined the crystal structure of Pyrococcus furiosus DNA polymerase (PfuPol) complexed with a cognate monomeric PCNA, which allowed us to construct a convincing model of the polymerase-PCNA ring interaction. Electron microscopy analyses confirmed that this complex structure exists among the multiple functional configurations in solution. Together with data from mutational analyses, this structural study indicated that the novel interaction between a stretched loop of PCNA and the PfuPol Thumb domain is quite important, in addition to the authentic PCNA-polymerase recognition site (PIP box). A comparison of the present structures with the previously reported structures of polymerases complexed with DNA suggested that the second interaction site plays a crucial role in switching between the polymerase and exonuclease modes, by stabilizing only the polymerase mode. This proposed mechanism of fidelity control of replicative DNA polymerases was supported by experiments, in which a mutation within the second interaction site caused an enhancement in the exonuclease activity in the presence of PCNA.
収録刊行物
-
- 日本結晶学会誌
-
日本結晶学会誌 52 (4), 201-207, 2010
日本結晶学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204087233024
-
- NII論文ID
- 10026599264
-
- NII書誌ID
- AN00188364
-
- COI
- 1:CAS:528:DC%2BC3cXhsFaltL%2FE
-
- ISSN
- 18845576
- 03694585
-
- NDL書誌ID
- 10832280
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可