Protein Crystal Processing Using Ultraviolet Laser

DOI Web Site 11 References
  • KITANO Hiroshi
    Core Technology Center, Nikon Corporation
  • ADACHI Hiroaki
    Department of Electrical Engineering, Graduate School of Engineering, Osaka University
  • MATSUMURA Hiroyoshi
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University
  • TAKANO Kazufumi
    Department of Material and Life Science, Graduate School of Engineering, Osaka University
  • INOUE Tsuyoshi
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University
  • MORI Yusuke
    Department of Electrical Engineering, Graduate School of Engineering, Osaka University
  • SASAKI Takatomo
    Department of Electrical Engineering, Graduate School of Engineering, Osaka University

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Other Title
  • 紫外レーザーを用いた蛋白質結晶の加工
  • サイキン ノ ケンキュウ カラ シガイ レーザー オ モチイタ タンパクシツ ケッショウ ノ カコウ

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Abstract

we proposed a new processing technique for protein crystals, pulsed UV laser soft ablation (PULSA) . Several kinds of protein crystals were successfully processed by laser irradiation at a wavelength of 193 nm. Unchanged resolution limit between pre- and post-processings in X-ray diffraction measurements showed PULSA had little influence on the crystallinity of the non-irradiated section. This technique will be a powerful tool for processing fragile biological-macromolecule crystals and will accelerate protein structural analysis.

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