Synthesis of Polyunsaturated Fatty Acid L-Menthyl Esters through Lipase-Catalyzed Esterification in an Organic Solvent-Free System
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- KOBAYASHI Takashi
- Osaka Municipal Technical Research Institute
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- NAGAO Toshihiro
- Osaka Municipal Technical Research Institute
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- KAWASHIMA Akiko
- Department of Food and Nutrition, Sonoda Women’s University
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- WATANABE Yomi
- Osaka Municipal Technical Research Institute
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- SHIMADA Yuji
- Osaka Municipal Technical Research Institute
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Abstract
Synthesis of L-menthyl eicosapentaenoate was achieved through lipase-catalyzed esterification of eicosapentaenoic acid (EPA) with L-menthol in an organic solvent-free system. A suitable enzyme was a lipase from Candida rugosa (Lipase-AY, Amano Enzyme Inc., Aichi) when L-menthol was esterified at 30°C in a mixture of L-menthol/EPA (1.2/1, mol/mol), 20 wt% water and 1600 U/g-mixture of lipase. The reaction reached equilibrium after 3 d at ca. 75% esterification. L-Menthyl γ-linolenate and arachidonate could also be synthesized under the same conditions as those in synthesis of L-menthyl eicosapentaenoate, although the degrees of esterification after 4 d were 54 and 41%, respectively. Synthesis of L-menthyl eicosapentaenoate greatly depended on the water content, and reaction rate significantly decreased in the presence of < 20% of water.<br>
Journal
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- Journal of Oleo Science
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Journal of Oleo Science 53 (6), 309-312, 2004
Japan Oil Chemists' Society
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Keywords
Details 詳細情報について
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- CRID
- 1390001204090734464
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- NII Article ID
- 10012946100
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- NII Book ID
- AA11503337
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- ISSN
- 13473352
- 13458957
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- NDL BIB ID
- 6943098
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed