Domain Formation and Phase Separation in Mixed Phospatidylcholine/Ceramide 3 Monolayers and Bilayers
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- IMURA Tomohiro
- Faculty of Science and Technology, Science University of Tokyo
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- SAKAI Hideki
- Faculty of Science and Technology, Science University of Tokyo Institute of Colloid and Interface Science, Science University of Tokyo
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- YAMAUCHI Hitoshi
- Faculty of Science and Technology, Science University of Tokyo
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- KAISE Chihiro
- Faculty of Science and Technology, Science University of Tokyo
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- MATSUMOTO Mutsuyoshi
- Nat'l. Inst. Master. Chem. Rers.
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- KOZAWA Kozo
- Faculty of Science and Technology, Science University of Tokyo
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- YOKOYAMA Shoko
- Kyoritsu College of Pharmacy
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- ABE Masahiko
- Faculty of Science and Technology, Science University of Tokyo Institute of Colloid and Interface Science, Science University of Tokyo
Bibliographic Information
- Other Title
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- フォスファチジルコリン/セラミド3混合単分子膜および混合二分子膜中でのドメイン形成と相分離
Description
Interactions between L-α-dipalmitoylphosphatidylcholine (DPPC) and 2S, 3S, 4R-2-stearoylamide-1, 3, 4-octadecanetriol (Ceramide 3) in mixed monolayers and bilayers were investigated by surface pressure measurements, atomic force microscopy (AFM) and differential scanning calorimetry (DSC).<BR>AFM images for mixed DPPC/Ceramide 3 monolayers (single-layer LB films) deposited at 10mN/m surface pressure on mica indicated elliptical domains. DSC curves indicated the phase transition temperature of DPPC liposomal bilayer membranes to shift upwards with the addition of Ceramide 3 and a sharp DPPC peak to become broad in mixed DPPC/Ceramide 3 systems. A DSC peak was noted to split at higher mole fractions of Ceramide 3.<BR>The phase separation was evident in mixed DPPC/Ceramide 3 monolayers and bilayers, and Ceramide 3 may possibly interact with DPPC in the liquid-expanded phase with consequent phase separation accompanied with domain formation.
Journal
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- Journal of Japan Oil Chemists' Society
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Journal of Japan Oil Chemists' Society 49 (4), 373-377,391, 2000
Japan Oil Chemists' Society
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Keywords
Details 詳細情報について
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- CRID
- 1390001204090926848
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- NII Article ID
- 130001014777
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- ISSN
- 18841996
- 13418327
- http://id.crossref.org/issn/13458957
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed