Effects of Steroids and Vitamin D<SUB>3</SUB> on the Permeability of Liposomal Bilayer Membranes

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  • ABE Masahiko
    Faculty of Science and Technology, Science University of Tokyo Institute of Colloid and Interface Science, Science University of Tokyo
  • HASHIZAKI Kaname
    Faculty of Science and Technology, Science University of Tokyo
  • YOKOUCHI Yumiko
    Faculty of Science and Technology, Science University of Tokyo
  • TAKAO Yoshiko
    Faculty of Science and Technology, Science University of Tokyo
  • MANOSROI Jiradei
    Faculty of Pharmacy, Chiang Mai University
  • MANOSROI Aranya
    Faculty of Pharmacy, Chiang Mai University
  • SAKAI Hideki
    Faculty of Science and Technology, Science University of Tokyo Institute of Colloid and Interface Science, Science University of Tokyo
  • YOKOYAMA Shoko
    Kyoritsu College of Pharmacy
  • YAMAUCHI Hitoshi
    Faculty of Science and Technology, Science University of Tokyo

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Other Title
  • リポソーム二分子膜の透過性に及ぼすステロイドとビタミンD<SUB>3</SUB>の影響
  • Effects of Steroids and Vitamin D3 on the Permeability of Liposomal Bilayer Membranes

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Molecular interactions between L-α-dipalmitoylphosphatidylcholine (DPPC) and steroids differing in the number of double bonds in a monolayer and bilayer were examined by measurement of surface pressure, permeability and microviscosity. The steroids used were 7-dehydrocholesterol (7-DHC), cholesterol (Chol) and β-cholestanol (β-Chol) along with Vitamin D3 (VD3) having an opened steroid B-ring. The limiting molecular area for the steroids in the DPPC-steroid mixed monolayer increased with the number of double bonds in the steroid ring in the order β-Chol<Chol<7-DHC. Liposomes were formed with steroids at steroid mole fractions less than 0.5 and VD3 less than 0.3. The permeability of the liposomes containing steroids decreased with the number of double bonds in the steroid ring and steroid limiting molecular area. That of liposomes containing VD3 with limiting molecular area exceeding that of steroids was higher than for liposomes containing steroids. The effects of steroids or VD3 on the permeability of Liposomal bilayer membranes cannot be adequately explained based on the number of double bonds or limiting molecular area, although Demel et al. have shown a correlation between the effects of sterols on the permeability of liposomes and molecular area of sterols in monolayers. The permeability of liposomal bilayer membranes containing steroids or VD3 was found in this study straightly decrease with microviscosity near the bilayer center. The permeability of liposomal bilayer membranes may thus be considered to related to microviscosity near the bilayer center, which can be evaluated based on fluorescence polarization determined with the fluorescent probe, 12AS.

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