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- Anjani Gemala
- Division of Material Sciences, Graduate School of Natural Science and Technology, Kanazawa University
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- Ohta Akio
- Division of Material Sciences, Graduate School of Natural Science and Technology, Kanazawa University
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- Yasuhara Kazuma
- Graduate School of Material Science, Nara Institute of Science and Technology
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- Asakawa Tsuyoshi
- Division of Material Sciences, Graduate School of Natural Science and Technology, Kanazawa University
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抄録
This study investigates the aggregation behavior of caseinate and the solubilization of genistein in aqueous caseinate solution. The critical aggregation concentration (CAC) of caseinate was obtained from the fluorescence intensity of 8-anilino-1-naphthalenesulfonic acid (ANS), which was enhanced by ANS-protein interactions and the hydrophobicity of caseinate. The increasing solubility of genistein in caseinate was confirmed by HPLC measurements; above and below the CAC, the genistein/caseinate molar ratio is 1:1 and 10:1, respectively. The latter ratio indicates that more caseinate molecules surround genistein below the CAC. However, the solubility of genistein in caseinate is unaffected by calcium ions. Atomic force microscopy (AFM) shows that casein sub-micelles are similarly structured in the presence and absence of genistein. In AFM phase images, the caseinate sub-micelle is brightened in the presence of genistein, implying that the particle becomes more rigid, probably because genistein attaches to the surface or to the narrow part of the sub-micelle. The diameter of sub-micelle aggregates is two times that of caseinate alone (24 nm versus 12 nm). These results were confirmed by cryo-TEM observations.
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 63 (4), 413-422, 2014
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204092187904
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- NII論文ID
- 130004436137
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- NII書誌ID
- AA11503337
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- COI
- 1:STN:280:DC%2BC2crgvF2iug%3D%3D
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 025629283
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- PubMed
- 24599106
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可