Comparison of γ-oryzanol contents in crude rice bran oils from different sources by various determination methods

DOI Web Site Web Site PubMed 被引用文献9件 参考文献7件 オープンアクセス
  • Yoshie Ayano
    Dept. of Materials and Life Science, Faculty of Science and Technology, Seikei University
  • Kanda Ayato
    Dept. of Materials and Life Science, Faculty of Science and Technology, Seikei University
  • Nakamura Takahiro
    Boso Oil & Fat Co. Ltd.
  • Igusa Hisao
    Boso Oil & Fat Co. Ltd.
  • Hara Setsuko
    Dept. of Materials and Life Science, Faculty of Science and Technology, Seikei University

書誌事項

タイトル別名
  • Comparison of .GAMMA.-Oryzanol Contents in Crude Rice Bran Oils from Different Sources by Various Determination Methods
  • Comparison of g oryzanol contents in crude rice bran oils from different sources by various determination methods
公開日
2009
DOI
  • 10.5650/jos.58.511
公開者
公益社団法人 日本油化学会

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説明

Although there are various determination methods for γ -oryzanol contained in rice bran oil by absorptiometry, normal-phase HPLC, and reversed-phase HPLC, their accuracies and the correlations among them have not been revealed yet. Chloroform-containing mixed solvents are widely used as mobile phases in some HPLC methods, but researchers have been apprehensive about its use in terms of safety for the human body and the environment.<BR>   In the present study, a simple and accurate determination method was developed by improving the reversed-phase HPLC method. This novel HPLC method uses methanol/acetonitrile/acetic acid (52/45/3 v/v/v), a non-chlorinated solvent, as the mobile phase, and shows an excellent linearity (y = 0.9527x + 0.1241, R2 = 0.9974) with absorptiometry. The mean relative errors among the existing 3 methods and the novel method, determined by adding fixed amounts of γ-oryzanol into refined rice salad oil, were -4.7% for the absorptiometry, -6.8% for the existing normal-phase HPLC, +4.6% for the existing reversed-phase HPLC, and -1.6% for the novel reversed-phase HPLC method. γ -Oryzanol content in 12 kinds of crude rice bran oils obtained from different sources were determined by the four methods. The mean content of those oils were 1.75±0.18% for the absorptiometry, 1.29±0.11% for the existing normal-phase HPLC, 1.51±0.10% for the existing reversed-phase HPLC, and 1.54±0.19% for the novel reversed-phase HPLC method.

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