Enzymatic Preparation of Human Milk Fat Substitutes and Their Oxidation Stability
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- Kotani Kousuke
- Faculty of Science and Technology, Seikei University
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- Yamamoto Yukihiro
- Faculty of Science and Technology, Seikei University
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- Hara Setsuko
- Faculty of Science and Technology, Seikei University
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抄録
Human milk fat substitutes (HMFSs), rich in palmitic acid (P) at the sn-2 position of triacylglycerol (TAG), were prepared from lard via Novozym435®- and Lipozyme RM-IM®-mediated two-step reactions. First, 2-palmitoyl monoacylglycerol (2-P-MAG, 90% purity) was prepared via Novozym435®mediated ethanolysis of lard. Then, 2-P-MAG, oleic acid (O), linoleic acid (L), and lard were dissolved in hexane and subjected to a Lipozyme RM-IM®-mediated reaction for HMFS preparation. The effect of the amount of 2-P-MAG and fatty acids (O and L) in HMFS preparation were investigated. Under the optimum reaction conditions: 7 mmol of lard, 3.0 mmol of 2-P-MAG, 5.2 mmol of O, 3.5 mmol of L, 10 mL of hexane, 10 wt% of Lipozyme RM-IMR® (against the total weight of substrates), 550 rpm, 37°C and 6 h, a HMFS with total fatty acid composition and P content at the sn-2 position of TAG similar to that of human milk fat was prepared. In the same way, a HMFS having polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (PUFA-HMFS) was prepared. The HMFS and PUFA-HMFS prepared in this study, as well as fats extracted from commercially available powdered milk for infants (FPM) were evaluated for their oxidation stability by an auto-oxidation test. The test showed HMFS and PUFA-HMFS to possess greater oxidation stability than that the FPMs. These results indicated that the HMFS and PUFA-HMFS prepared in this study have value as potential ingredients for powdered milk.
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 64 (3), 275-281, 2015
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204092714112
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- NII論文ID
- 130004800111
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- NII書誌ID
- AA11503337
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 026160348
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- PubMed
- 25757431
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可