Isolation of cytotoxic glucoerebrosides and long-chain bases from sea cucumber Cucumaria frondosa using high speed counter-current chromatography
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- Xu Jie
- College of Food Science and Engineering, Ocean University of China
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- Guo Shuang
- College of Food Science and Engineering, Ocean University of China
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- Du Lei
- College of Food Science and Engineering, Ocean University of China
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- Wang Yu-Ming
- College of Food Science and Engineering, Ocean University of China
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- Sugawara Tatsuya
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Hirata Takashi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Xue Chang-Hu
- College of Food Science and Engineering, Ocean University of China
書誌事項
- タイトル別名
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- Isolation of cytotoxic glucoerebrosides and long-chain bases from sea cucumber <i>Cucumaria frondosa</i> using high speed counter-current chromatography
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抄録
Total glucocerebrosides of the sea cucumber Cucumaria frondosa (CFC) have been isolated from the less polar lipid fraction of the chloroform–methanol extract using high speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether-methanol-water (5:4:1, v/v). Three glucocerebroside molecular species (CFC-1, CFC-2 and CFC-3) were isolated from crude total cerebrosides with repeated column chromatography. The structures of these three glucocerebroside molecular species were determined on the basis of chemical and spectroscopic evidence: fatty acids were mainly saturated (C22:0 and C18:0), monounsaturated (C24:1 and C20:1) and α-hydroxyl fatty acids (C24:1h, C23:0h, C23:1h and C22:0h), the structures of long-chain bases were dihydroxy (d17:1, d18:2 and d18:1) and trihydroxy (t17:0 and t16:0), and the glycosylation was glucose. High purity long-chain bases of sea cucumber Cucumaria frondosa (CF-LCB) were prepared from total lipids by HSCCC with a two-phase solvent system composed of n-hexane-methyl tert butyl ether-methanol-water (1:1:2:1, v/v). Compare with traditional preparative methods, the method of HSCCC is short cycle, high yield and less solvent consumption. The composition analysis of CF-LCB showed that the ratio of d18:2 and d17:1 was approximately 2:1. The four glucocerebrosides and long-chain bases from sea cucumber Cucumaria frondosa were evaluated for activity in vitro assays for the cytotoxic activities against Caco-2 colon cancer cells. The results indicated that both glucocerebrosides and long-chain bases exhibited an inhibitory effect on cell proliferation. Moreover, CFC-3 was most effective in four glucocerebrosides to Caco-2 cell viability. The inhibition effect of CF-LCB was much stronger than glucocerebrosides.
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 62 (3), 133-142, 2013
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204092843392
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- NII論文ID
- 130004436023
- 40019582130
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- NII書誌ID
- AA11503337
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- COI
- 1:CAS:528:DC%2BC3sXjsFKis7s%3D
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 024272481
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- PubMed
- 23470440
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可