Fractionation of Conjugated Linoleic Acid Isomers by Selective Hydrolysis with Candida rugosa Lipase
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- YAMAUCHI-SATO Yoshie
- Rinoru Oil Mills Co. Ltd.
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- NAGAO Toshihiro
- Osaka Municipal Technical Research Institute
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- YAMAMOTO Takaya
- Rinoru Oil Mills Co. Ltd.
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- TERAI Tadamasa
- Department of Applied Chemistry, Osaka Institute of Technology
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- SUGIHARA Akio
- Environmental and Systems Engineering, Faculty of Engineering, Tokushima Bunri University
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- SHIMADA Yuji
- Osaka Municipal Technical Research Institute
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抄録
It was attempted to prepare cis-9, trans-11 conjugated linoleic acid (c9,t11-CLA) and t10,c12-CLA concentrates that can be used as foods. A free fatty acid mixture (FFA-CLA) containing almost equal amounts of c9,t11- and t10,c12-CLAs was esterified with glycerol using immobilized Rhizomucor miehei lipase, and the resulting acylglycerols (Gly-CLA) were purified by molecular distillation. Contents of c9,t11- and t10,c12-CLAs in Gly-CLA were the same as those in FFA-CLA: c9,t11-CLA, 33.7 wt%; t10,c12-CLA, 34.5 wt%. Gly-CLA was first hydrolyzed with an equal weight of water and 1.0 U/g-mixture of Candida rugosa lipase, and c9,t11-CLA-rich FFAs were prepared by molecular distillation: purity of c9,t11-CLA based on the total content of c9,t11- and t10,c12-CLAs, 72.9%. Meanwhile, purity of t10,c12-CLA in acylglycerols was 65.0%. To further increase the purity, the acylglycerols were hydrolyzed again with 15 U/g-mixture of C. rugosa lipase, resulting in enrichment of t10,c12-CLA in acylglycerols (purity of t10,c12-CLA, 80.4%). Non-selective hydrolysis of t10,c12-CLA-rich acylglycerols with 200 U/g-mixture of C. rugosa lipase produced t10,c12-CLA-rich FFAs (purity of t10,c12-CLA, 81.5%). In addition, c9,t11-CLA-rich FFAs were successfully esterified with glycerol using immobilized R. miehei lipase, and c9,t11-CLA-rich acylglycerols can be synthesized (purity of c9,t11-CLA, 73.0%). The process was composed of reactions with C. rugosa and R. miehei lipases, which can be used for production of foods, and molecular distillation. Hence, the c9,t11- and t10,c12-CLA concentrates can be used as foods.<br>
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 52 (7), 367-374, 2003
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204092906880
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- NII論文ID
- 130000060496
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- NII書誌ID
- AA11503337
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- COI
- 1:CAS:528:DC%2BD3sXkvFCku78%3D
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 6567509
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可