Identification of a Novel Mutation in FAD2B from a Peanut EMS Mutant with Elevated Oleate Content
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- Fang Chao Qi
- Shandong Peanut Research Institute
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- Wang Chuan Tang
- Shandong Peanut Research Institute
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- Wang Pi Wu
- Jilin Agricultural University
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- Tang Yue Yi
- Shandong Peanut Research Institute
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- Wang Xiu Zhen
- Shandong Peanut Research Institute
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- Cui Feng Gao
- Shandong Peanut Research Institute
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- Yu Shan Lin
- Shandong Peanut Research Institute
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Description
A Virginia type peanut mutant with more than 60% oleate content (E2-4-83-12) was selected from an EMS mutagenized population of LF2 (an export type peanut cultivar with 44.2% oleate) by near infrared reflectance spectroscopy. Cloning and sequencing of FAD2B from LF2 and E2-4-83-12 identified a novel mutation (C313T in the coding region) causing an H105Y substitution in the first histidine box of the FAD2B protein. GC-MS analysis of fatty acids in yeast cells harboring pYES2 with the mutated FAD2B detected no linoleate, confirming that FAD2B from E2-4-83-12 was dysfunctional. Loss-of-function FAD2A and FAD2B together contributed to elevated oleate phenotype of the peanut EMS mutant.
Journal
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- Journal of Oleo Science
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Journal of Oleo Science 61 (3), 143-148, 2012
Japan Oil Chemists' Society
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Details 詳細情報について
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- CRID
- 1390001204093233024
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- NII Article ID
- 130001882218
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- NII Book ID
- AA11503337
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- COI
- 1:STN:280:DC%2BC383lvFyqtQ%3D%3D
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- ISSN
- 13473352
- 13458957
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- NDL BIB ID
- 023425024
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- PubMed
- 22362145
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed