Detection of Genes Involved in Fatty Acid Elongation and Desaturation in Thraustochytrid Marine Eukaryotes
-
- Nagano Naoki
- Department of Marine Biology and Environmental Sciences, Faculty of Agriculture, University of Miyazaki
-
- Sakaguchi Keishi
- Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
-
- Taoka Yousuke
- Nippon Suisan Kaisha Ltd.
-
- Okita Yuji
- Nippon Suisan Kaisha Ltd.
-
- Honda Daiske
- Department of Biology, Faculty of Science and Engineering, Konan University
-
- Ito Makoto
- Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University Bio-Architecture Center, Kyushu University
-
- Hayashi Masahiro
- Department of Marine Biology and Environmental Sciences, Faculty of Agriculture, University of Miyazaki
この論文をさがす
抄録
Heterotrophic marine protists known as thraustochytrids can synthesize polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA). The biosynthetic pathways of PUFAs in thraustochytrids are poorly understood, however. In this study, we attempted to reveal the enzymes involved in DHA synthesis in thraustochytrids. Nine thraustochytrid strains representing 3 genera (Aurantiochytrium, Schizochytrium, and Thraustochytrium) were used for PCR-based detection of the genes encoding Δ5-elongase and Δ4-desaturase and for fatty acid analysis. The degenerate primers were designed to amplify the Δ5-elongase and Δ4-desaturase genes, and the partial sequences of the enzymes were obtained from the genera Thraustochytrium and Schizochytrium. These fragments were identical to those of known Δ5-elongase and Δ4-desaturase. Neither Δ5-elongase nor Δ4-desaturase was detected in the strains belonging to the genus Aurantiochytrium, however, suggesting that this group likely synthesizes DHA not via the elongation/desaturation pathway but via an alternate pathway such as the polyketide synthase pathway. The fatty acid profiles of thraustochytrids were consistent with the presence of genes involved in PUFA biosynthesis in thraustochytrid genera. Thus, our findings suggest that two biosynthetic pathways for PUFAs exist in these organisms.
収録刊行物
-
- Journal of Oleo Science
-
Journal of Oleo Science 60 (9), 475-481, 2011
公益社団法人 日本油化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204093536896
-
- NII論文ID
- 130001008604
-
- NII書誌ID
- AA11503337
-
- ISSN
- 13473352
- 13458957
-
- NDL書誌ID
- 11201868
-
- PubMed
- 21852747
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可