3D-Human Tissue Chips Fabricated by Inkjet Cell Printing

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  • MATSUSAKI Michiya
    Department of Applied Chemistry, Graduate School of Engineering, Osaka University
  • AKASHI Mitsuru
    Department of Applied Chemistry, Graduate School of Engineering, Osaka University

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We demonstrate a LbL assembly using the inkjet printing of single cells and proteins to enable the fabrication of 3D human micro-tissue arrays, which are integrated structures of micrometer-sized cellular multilayers controlled at the single-cell layer level, for application of pharmaceutical assays. Introduction of automatic inkjet printing of cells and proteins must be necessary for giving an assurance of their reproducibility. The 440micro-arrays which were the integration of simplified and multilayered liver structures were successfully developed and comprehensive high-throughput assays of liver functions were performed. We found for the first time that these simplified 3D liver structures, a sandwich of endothelial cells and hepatocytes, revealed the highest functions as compared to other 3D-structures. These 3D-human tissue chips would enable the “total-human tissue models” for tailor-made drug screening.

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  • 日本画像学会誌

    日本画像学会誌 51 (5), 524-530, 2012

    一般社団法人 日本画像学会

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