Cytogenetic Analysis of A- and B-Chromosomes of Rhamdia hilarii (Teleostei, Pimelodidae): C-Banding, Silver Nitrate and CMA3 Staining and Restriction Endonuclease Banding.
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- Maistro Edson Luis
- Instituto de Farmácia e Nutricão, UNIFENAS
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- Oliveira Claudio
- Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista
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- Foresti Fausto
- Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista
説明
We investigated the chromosome complement of Rhamdia hilarii using C-banding, silver nitrate and CMA3 staining, and chromosome digestion with 9 restriction endonucleases (RE). The results revealed that this species has 2n=58 to 2n=60 chromosomes, with the numerical variation being due to the presence of 1 or 2 metacentric B-chromosomes. The C-banding pattern showed a small amount of heterochromatin in A-chromosomes and heterochromatin in both telomeric regions of B-chromosomes. The Ag-NOR and CMA3 staining revealed that the rDNA loci were located in the terminal position on the short arm of a pair of submetacentric chromosomes. The heterochromatin of the B-chromosomes was not stained brightly with CMA3 showing that these regions segments are not GC-rich. Chromosome digestion with different REs permitted the identification of homologous chromosome pairs in some cases. On the basis of the results, we discuss some aspects of chromosome structure and the origin of the B-chromosomes in Rhamdia.
収録刊行物
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- CYTOLOGIA
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CYTOLOGIA 67 (1), 25-31, 2002
公益財団法人 日本メンデル協会
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詳細情報 詳細情報について
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- CRID
- 1390001204104904576
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- NII論文ID
- 130004447774
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- ISSN
- 13487019
- 00114545
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可