Fluorescent Labeling of the Cyst Nematode <i>Heterodera glycines</i> in Deep-Tissue Live Imaging
-
- Ohtsu Mina
- Graduate School of Science, Nagoya University
-
- Kurihara Daisuke
- Graduate School of Science, Nagoya University JST ERATO Higashiyama Live-Holonics Project, Nagoya University
-
- Sato Yoshikatsu
- Institute of Transformative Bio-Molecules (ITbM), Nagoya University
-
- Suzaki Takuya
- Graduate School of Life and Environmental Sciences, University of Tsukuba
-
- Kawaguchi Masayoshi
- Division of Symbiotic Systems, National Institute for Basic Biology (NIBB)
-
- Maruyama Daisuke
- Kihara Institute for Biological Research, Yokohama City University
-
- Higashiyama Tetsuya
- Graduate School of Science, Nagoya University JST ERATO Higashiyama Live-Holonics Project, Nagoya University Institute of Transformative Bio-Molecules (ITbM), Nagoya University
説明
<p>Nematode infection of plant roots is a paradigm of host–parasite interactions. Although nematodes can be labeled with fluorescent dyes, migration of the worms into the deep regions of host roots makes them difficult to track. Here we report the use of two fluorescent dyes, FM4-64 and SYBR green I, to intensely label the soybean cyst nematode (SCN) Heterodera glycines for one week in host plants. Continuous monitoring of the labeled SCN juveniles was achieved with two-photon microscopy. Additionally, we developed a transient transformation system consisting of the non-model leguminous plant (fabaceous) roots, Astragalus sinicus and Agrobacterium rhizogenes to observe the cellular structures of the plant during SCN infection. By the combined use of fluorescent dyes and two-photon microscopy, clear images of infecting SCNs were obtained even in deep regions of A. sinicus roots. The fluorescent labeling described herein can also be used in detailed monitoring of the infection processes of other non-model nematodes, as well as the associated morphological changes in the host plant roots.</p>
収録刊行物
-
- CYTOLOGIA
-
CYTOLOGIA 82 (3), 251-259, 2017
公益財団法人 日本メンデル協会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204109032448
-
- NII論文ID
- 130006068235
-
- ISSN
- 13487019
- 00114545
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可