Cyto-Molecular Characterization of Three <i>Tacca</i> Species from Bangladesh
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- Hossen Riyad
- Department of Botany, University of Dhaka
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- Sultana Syeda Sharmeen
- Department of Botany, University of Dhaka
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- Alam Sheikh Shamimul
- Department of Botany, University of Dhaka
説明
<p>Three species of Tacca J. R. & G. Forst., namely T. plantaginea, T. chantrieri and T. integrifolia, were investigated cytogenetically and at the molecular level to solve their taxonomic rank. The diploid chromosome number 2n=28 was found in T. plantaginea and T. integrifolia whereas 2n=22 was found in T. chantrieri. This is probably the first report on the diploid chromosome number of T. plantaginea and T. chantrieri around the world. The three species differed in respect of centromeric formulae such as 22m+6sm in T. plantaginea, 18m+4sm in T. chantrieri and 28m in T. integrifolia. In addition to regular 2n=22 chromosomes, four to eight small chromosomes were found in some cells of T. chantrieri suggesting the probable occurrence of B-chromosome. In T. integrifolia, a pair of satellites was found on the short arm, one in each member of pair VI, after orcein and CMA staining but not in DAPI, revealing the GC-rich nature. A wide range of CMA- and DAPI-positive bands was found in the metaphase chromosomes of the three species. DAPI-fluoresced chromosomes were frequent in these three species. Six primer combinations were applied for RAPD analysis in the three species of Tacca to find out their genomic relationship. The three species showed some unique bands useful as markers for each species. Conventional and fluorescent karyotype together with RAPD fingerprinting indicated that T. integrifolia is distantly related to T. plantaginea and T. chantrieri. Therefore, the compilation of the above cytological and molecular information will be very useful for authentic identification of the three Tacca species. This genetic information will help in the proper conservation of and to make patent for the three threatened Tacca species found in Bangladesh.</p>
収録刊行物
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- CYTOLOGIA
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CYTOLOGIA 81 (4), 371-377, 2016
公益財団法人 日本メンデル協会
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詳細情報 詳細情報について
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- CRID
- 1390001204110385024
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- NII論文ID
- 130005187636
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- ISSN
- 13487019
- 00114545
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可