Genome Size of the Ultrasmall Unicellular Freshwater Green Alga, Medakamo hakoo 311, as Determined by Staining with 4′,6-Diamidino-2-phenylindole after Microwave Oven Treatments: II. Comparison with Cyanidioschyzon merolae, Saccharomyces cerevisiae (n, 2n), and Chlorella variabilis
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- Kuroiwa Tsuneyoshi
- Department of Chemical and Biological Science, Faculty of Science, Japan Women’s University Core Research for Evolutional Science and Technology, Japan Science and Technology Agency
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- Ohnuma Mio
- Core Research for Evolutional Science and Technology, Japan Science and Technology Agency Institute of Technology, Hiroshima College
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- Imoto Yuuta
- Core Research for Evolutional Science and Technology, Japan Science and Technology Agency Medical Institute of Bioregulation, Kyushu University
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- Misumi Osami
- Core Research for Evolutional Science and Technology, Japan Science and Technology Agency Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University
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- Nagata Noriko
- Department of Chemical and Biological Science, Faculty of Science, Japan Women’s University
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- Miyakawa Isamu
- Department of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University
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- Fujishima Masahiro
- Department of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University
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- Yagisawa Fumi
- Instrumental Research Center, University of the Ryukyus
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- Kuroiwa Haruko
- Department of Chemical and Biological Science, Faculty of Science, Japan Women’s University Core Research for Evolutional Science and Technology, Japan Science and Technology Agency
書誌事項
- タイトル別名
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- Genome Size of the Ultrasmall Unicellular Freshwater Green Alga, <i>Medakamo hakoo</i> 311, as Determined by Staining with 4′,6-Diamidino-2-phenylindole after Microwave Oven Treatments: II. Comparison with <i>Cyanidioschyzon merolae</i>, <i>Saccharomyces cerevisiae</i> (<i>n</i>, 2<i>n</i>), and <i>Chlorella variabilis</i>
抄録
Eukaryotic cells contain three types of nuclei: the cell nucleus, the mitochondrial nuclei (nucleoids), and plastid nuclei (nucleoids). Here, we report that the chlorophyte strain Medakamo hakoo 311 has the smallest cell nucleus genome among the free-living eukaryotes analyzed to date. The size of the nuclear genome was analyzed by staining with 4′,6-diamidino-2-phenylindole (DAPI), followed by fluorimetry using a video-intensified microscope photon-counting system (VIMPCS) and fluorescence microscopy. The exact genome size of M. hakoo remains elusive because of the low permeability of the thick algal cell wall to DAPI. To improve the DAPI staining method for M. hakoo, we added a microwave oven heating step. We examined the genome sizes of M. hakoo, Cyanidioschyzon merolae, Saccharomyces cerevisiae (n, 2n), and Chlorella variabilis 1 N by DAPI staining, which included a microwave oven heating step, followed by fluorimetry with the VIMPCS. The new method of DAPI staining that included a microwave oven heating step improved the staining efficiency of C. variabilis and M. hakoo nuclei, and slightly improved the staining efficiency of S. cerevisiae and C. merolae nuclei. Using well-stained samples and the cell nucleus genome size of C. merolae (16.5 Mb) as the standard, the cell nucleus genome sizes of M. hakoo, S. cerevisiae (n), and C. variabilis were determined as 9.2, 20.2, and 35.9 Mb, respectively. The results are consistent with previous reports that M. hakoo cell has the smallest genome among the free-living eukaryotes evaluated to date.
収録刊行物
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- CYTOLOGIA
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CYTOLOGIA 81 (1), 69-76, 2016
公益財団法人 日本メンデル協会
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詳細情報 詳細情報について
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- CRID
- 1390001204110918912
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- NII論文ID
- 130005143755
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- ISSN
- 13487019
- 00114545
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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