Analysis of DNA damawge and AhR ligand activity in treated sewage effluent by using HPLC-bioassay

OTOBE Fumiko, CHOU Pei-Hsin, MATSUI Saburo, ODA Yoshimitu, MATSUDA Tomanari

doi:10.11532/proes1992.43.113

Bibliographic Information

Other Title

ＨＰＬＣ‐バイオアッセイを用いた下水処理水中のＤＮＡ損傷性およびＡｈＲリガンド活性の解析

Description

In treated sewage effluents, DNA damage toxicity and endocrine disrupting activity have been detected. But the responsible toxic substances have not fully been identified.<BR>In this study, we concentrated treated sewage effluents at Kyoto city by Sep-Pak C18 cartridges and eluted with methanol and acetone. The 50% methanol extract and the 100% methanol-acetone extract were fractionated by HPLC and three bioassays including umu test, a Bacillus subtilis rec-assay and a yeast AhR reporter gene assay were applied to each fraction. The results showed different patterns in three bioassays. By using umu test, the activity of DNA damage toxicity was detected in fractions of the 50% methanol extract. The Bacillus subtilis rec-assay and the yeast AhR reporter gene assay indicated DNA damage toxicity and AhR ligand activity in different fractons of the 100% methanol-acetone extract. Furthermore, Acid Blue 9 classified into acid dye was contained within the 50% methanol extract fraction. But DNA damage toxicity of Acid Blue 9 was not detected by umu test.

Journal

ENVIRONMENTAL ENGINEERING RESEARCH

ENVIRONMENTAL ENGINEERING RESEARCH 43 113-118, 2006

Japan Society of Civil Engineers

Keywords

Details 詳細情報について

CRID: 1390001204111472896

NII Article ID: 130003949504

DOI: 10.11532/proes1992.43.113

ISSN: 1884829X; 13415115

Text Lang: ja

Data Source

JaLC
CiNii Articles

Abstract License Flag: Disallowed

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