藻類増殖阻害試験における生物量の測定方法が毒性評価に及ぼす影響

DOI

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タイトル別名
  • Effect of Biomass Measurements on Toxicity Evaluation in Algal Growth Inhibition Tests.

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説明

General methods for the biomass measurements in algal growth inhibition tests are cell counting, absorbance, and fluorescence, but a standard method is not restricted by the standard procedures. The possibilityof the different evaluation is present in the toxicity test by different biomass determinations.In this study, the toxicity of copper (Cu) and monochloramine (NH2Cl) as reference toxic substances wereevaluated from the algal growth inhibition test using green alga Selenasturum capricornutum. To determine thebiomass changes, direct cell counting, turbidity, chlorophyll with fluorescence, and ATP methods were used andthe effective concentration of Cu and NH2Cl were evaluated with each results. The most sensitive method of theevaluation was direct cell counting, then LOEC and EC50 of Cu were 10 and 7 μg/l, and LOEC and EC50 of NH2Cl were 7 and 19 μg-Cl2/l, respectively. Turbidity and fluorescence methods showed the same sensitivity for Cu, whereas these were not sensitive for NH2Cl. In contrast, the sensitivity of ATP method was not good for bothtoxic substances, and the evaluated values of LOEC and EC50 with ATP were several times higher than withcounting method. It is necessary to recognize that the method for biomass measurements in algal growth test hasan effect on the estimation.

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詳細情報 詳細情報について

  • CRID
    1390001204112561280
  • NII論文ID
    130003775740
  • DOI
    10.11532/proes1992.35.101
  • ISSN
    1884829X
    13415115
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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