Competitive Immunoassay Using Capillary Electrophoresis with a Chemiluminescence Detector
-
- Tsukagoshi Kazuhiko
- Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
-
- Jinno Naoya
- Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
-
- Toguchi Kae
- Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
-
- Nakajima Riichiro
- Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
この論文をさがす
抄録
We have proposed competitive immunoassay using capillary electrophoresis (CE) with a chemiluminescence (CL) detector, in which a small amount of sample (ca. 20 μL) is required for analysis. Human serum albumin (HSA) and anti-human serum IgG (anti-HSA) were used for immune reaction as a model. A luminol and hydrogen peroxide CL reaction was adopted, and HSA was labeled with isoluminol isothiocyanate (ILITC) for competitive immunoassay. The reactant after the immune reaction was directly subjected to CE with the CL detector, where the labeled HSA was easily and rapidly separated and detected. The amount of labeled HSA indicated a good relationship to that of HSA as an analyte through the immune reaction. The HSA was determined over the range of 0.2–1.2 μM with a detection limit of 0.1 μM (S/N = 3). The present method features high sensitivity, a small sample volume, and easy and rapid operation. The method also shows the possibility to analyze a specific protein in a serum sample.
収録刊行物
-
- Bulletin of the Chemical Society of Japan
-
Bulletin of the Chemical Society of Japan 78 (10), 1791-1794, 2005
公益社団法人 日本化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204130085120
-
- NII論文ID
- 130004151826
- 40006898730
-
- NII書誌ID
- AA00580132
-
- ISSN
- 13480634
- 00092673
-
- NDL書誌ID
- 7468916
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可