Pigments and Chromogens used in Clinical Laboratory Testing

DOI Web Site Web Site 1 References

Bibliographic Information

Other Title
  • 臨床化学検査で用いられる色素と色原体
  • リンショウ カガク ケンサ デ モチイラレル シキソ ト イロ ゲンタイ

Search this article

Abstract

<p>Since the measurement method that is most commonly used in clinical chemistry tests is absorptiometry, pigments and chromogens are widely used as reagents for the measurement. In the electrophoresis of serum proteins, Ponceau S and Ponceau 3R are used as the dyeing pigments of albumin and of alpha1, alpha2, beta, and gamma globulins that are separated on a supporting medium. In addition, in the measurement of many serum components, such as total cholesterol and uric acid, chromogens including 4-aminoantipyrine and phenol are used as reagents in the presence of oxidized peroxidase. Furthermore, in the measurement of enzyme activity, a two- or three-stage conjugate reaction system is assembled in order to detect it by absorptiometry. Since reagents are used in large quantities in clinical chemistry tests, it is absolutely essential that they possess no toxicity or carcinogenicity and do not require any special waste fluid processing. There exist coexisting pigment substances such as bilirubin and oxygenated hemoglobin. Dual wavelength photometry is carried out to negate the influence of these coexisting substances, but it is necessary that the absorption bands between reaction products and coexisting substances are different. Therefore, a reaction system that has an absorption band in the long wavelength region and provides stable color development has been studied.</p>

Journal

References(1)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top